Anti-Cathepsin D/Ctsd Antibody

• Catalog Number: orb412968
• Category: Antibodies
• Description: Anti-Cathepsin D/Ctsd Antibody. Tested in ELISA, IF, ICC, and WB applications. This antibody reacts with Mouse and Rat, offering an essential tool for scientific applications such as cell lysate analysis and breast adenocarcinoma cell line research.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Immunogen: E. coli-derived mouse Cathepsin D recombinant protein (Position: K161-Q358).
• UniProt ID: P18242
• MW: 45 kDa
• Tested applications: ELISA, ICC, IF, WB
• Application notes: Western blot,0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 5 μg/ml ELISA,0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cathepsin D; Ctsd
• Note: For research use only

Western blot analysis of Cathepsin D using anti-Cathepsin D antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates, Lane 2: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hours at RT. The membrane was incubated with rabbit anti-Cathepsin D antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times for 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cathepsin D at approximately 45KD. The expected band size for Cathepsin D is at 45KD.