Caspase 3 Rabbit Polyclonal Antibody

SKU: orb213644

Description

The Caspase 3 Antibody [KO/KD Validated] is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Reactivity: Bovine, Canine, Human, Monkey, Mouse, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
• Target: CASP3
• Molecular Weight: Precursor 31 kDa, cleaved forms 17~25 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Immunofluorescent analysis of Caspase 3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunohistochemical analysis of paraffin-embedded human lung cancer using Caspase 3 Antibody orb213644 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded mouse brain using Caspase 3 Antibody orb213644 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat lymph node using Caspase 3 Antibody orb213644 with dilution at 1:100.

Western blot analysis of mouse brain (1), 293T (2) using Caspase 3 Antibody orb213644 with dilution at 1:1000.

Quick Database Links

Gene Symbol

CASP3

UniProt

P42574, P55213, P70677

NCBI Gene ID

12367, 25402, 836