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Catalog Number | orb443227 |
---|---|
Category | Antibodies |
Description | Anti-Cardiac Troponin T/TNNT2 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human Cardiac Troponin T recombinant protein (Position: K88-K298). |
Antibody Type | Primary Antibody |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 43 kDa |
UniProt ID | P45379 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Troponin T, cardiac muscle; TnTc; Cardiac muscle t Read more... |
Note | For research use only |
Application notes | Western blot, 0.1-0.5μg/ml ELISA, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Cardiac Troponin T using anti-Cardiac Troponin T antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin T antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac Troponin T at approximately 43KD. The expected band size for Cardiac Troponin T is at 36KD.
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
iFluor647 |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PE |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
APC |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
FITC |