Cardiac FABP/Fabp3 Antibody

SKU: orb402472

Description

Anti-Cardiac FABP/Fabp3 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western blot, 0.1-0.5μg/ml ELISA (Cap), 1-5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived rat Cardiac FABP recombinant protein (Position: A2-A133).
• Molecular Weight: 15 kDa
• Purification: Immunogen affinity purified.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Fabp3

Sandwich ELISA - Recombinant rat Cardiac FABP/Fabp3 protein standard curve. Use in combination with reagents from Rat Cardiac FABP/Fabp3 ELISA Kit EZ-Set (DIY Antibody Pairs).

Western blot analysis of Cardiac FABP/Fabp3 using anti-Cardiac FABP/Fabp3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP/Fabp3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac FABP/Fabp3 at approximately 15 kDa. The expected band size for Cardiac FABP/Fabp3 is at 15 kDa.

Quick Database Links

UniProt

P07483