CARD4/NOD1 Rabbit Polyclonal Antibody

SKU: orb669193

Description

Anti-CARD4/NOD1 Antibody. Tested in WB applications. This antibody reacts with Monkey.

Research Area

Disease Biomarkers, Immunology & Inflammation

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Monkey
• Reactivity: Monkey

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CARD4/NOD1, which shares 88.2% and 93.8% amino acid (aa) sequence identity with mouse and rat CARD4/NOD1, respectively.
• Target: Nucleotide-binding oligomerization domain-containing protein 1
• Molecular Weight: 108 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

nucleotide binding oligomerization domain containing 1; CARD4; CLR7.1; NLRC1; NOD1

Western blot analysis of CARD4/NOD1 using anti-CARD4/NOD1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates, Lane 2: monkey skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD4/NOD1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CARD4/NOD1 at approximately 108 KD. The expected band size for CARD4/NOD1 is at 108 KD.

Quick Database Links

Gene Symbol

Nucleotide-binding oligomerization domain-containing protein 1

UniProt

Q9Y239