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Anti-Carbonic Anhydrase 9/CA9 Antibody

Catalog Number: orb1173500

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb1173500
CategoryAntibodies
DescriptionAnti-Carbonic Anhydrase 9/CA9 Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, IHC, WB
ReactivityHuman, Mouse
IsotypeRabbit IgG
ImmunogenE.coli-derived human Carbonic Anhydrase 9/CA9 recombinant protein (Position: D146-D414).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW54 kDa
UniProt IDQ16790
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesMyosin-binding protein C, cardiac-type; Cardiac My
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NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human, Mouse ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-Carbonic Anhydrase 9/CA9 Antibody

Flow Cytometry analysis of RAW264.7 cells using anti-Carbonic Anhydrase 9/CA9 antibody. Overlay histogram showing RAW264.7 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-Carbonic Anhydrase 9/CA9 Antibody

Flow Cytometry analysis of U87 cells using anti-Carbonic Anhydrase 9/CA9 antibody. Overlay histogram showing U87 cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-Carbonic Anhydrase 9/CA9 Antibody

IHC analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody. Carbonic Anhydrase 9/CA9 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Carbonic Anhydrase 9/CA9 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-Carbonic Anhydrase 9/CA9 Antibody

IHC analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody. Carbonic Anhydrase 9/CA9 was detected in a paraffin-embedded section of human renal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Carbonic Anhydrase 9/CA9 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-Carbonic Anhydrase 9/CA9 Antibody

IHC analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody. Carbonic Anhydrase 9/CA9 was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Carbonic Anhydrase 9/CA9 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-Carbonic Anhydrase 9/CA9 Antibody

Western blot analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase 9/CA9 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase 9/CA9 at approximately 54 kDa. The expected band size for Carbonic Anhydrase 9/CA9 is at 50 kDa.

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    ELISA,  FC,  IHC,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-Carbonic Anhydrase 9/CA9 Antibody [orb2594333]

    ELISA,  FC,  IHC,  WB

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    100 μg
  • Anti-Carbonic Anhydrase 9/CA9 Antibody [orb2594334]

    ELISA,  FC,  IHC,  WB

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  • Anti-Carbonic Anhydrase 9/CA9 Antibody [orb2594335]

    ELISA,  FC,  IHC,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    HRP

    100 μg
  • Anti-Carbonic Anhydrase 9/CA9 Antibody [orb2594336]

    ELISA,  FC,  IHC,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    FITC

    100 μg