Anti-Carbonic Anhydrase 5A Antibody

• Catalog Number: orb213628
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CA5A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Carbonic Anhydrase 5A. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CA5A
• Entrez: 763, 12352, 54233
• UniProt ID: P23589, P43165, P35218
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti CA5 antibody, anti Carbonic anhydrase 5A, mit
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Carbonic Anhydrase 5A expression in HEK293T (A), mouse liver (B), rat liver (C), rat spleen (D) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 30 kD)

Immunofluorescent analysis of Carbonic Anhydrase 5A staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).