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Anti-Calcitonin/CALCA Antibody

Catalog Number: orb371671

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb371671
CategoryAntibodies
DescriptionAnti-Calcitonin/CALCA Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIHC
ReactivityMouse, Rat
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminus of mouse Calcitonin, different from the related human sequence by four amino acids, and identical to the related rat sequence.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW75 kDa, 45 kDa
UniProt IDQ99JA0
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCalcitonin gene-related peptide 1; Alpha-type CGRP
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NoteFor research use only
Application notesImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat . Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-Calcitonin/CALCA Antibody

IHC analysis of Calcitonin using anti-Calcitonin antibody. Calcitonin was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Calcitonin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-Calcitonin/CALCA Antibody

IHC analysis of Calcitonin using anti-Calcitonin antibody. Calcitonin was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Calcitonin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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