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Anti-Calcitonin/CALCA Antibody

Catalog Number: orb371670

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb371670
CategoryAntibodies
DescriptionAnti-Calcitonin/CALCA Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIHC
ReactivityHuman
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminus of human Calcitonin, different from the related mouse and rat sequences by four amino acids.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW75 kDa, 45 kDa
UniProt IDP06881
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCalcitonin gene-related peptide 1; Alpha-type CGRP
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NoteFor research use only
Application notesImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human . Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-Calcitonin/CALCA Antibody

IHC analysis of Calcitonin using anti-Calcitonin antibody. Calcitonin was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Calcitonin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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