c-FOS Rabbit Polyclonal Antibody

SKU: orb1289737

Description

The c-FOS Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant protein corresponding to human c-FOS. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-1:1000, IF:IC: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein corresponding to human c-FOS.
• Target: FOS
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

G0S7; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7

Western blot analysis of c-FOS expression in Hela treated by PMA/TPA (A) whole cell lysates. (Predicted band size: 28; 36; 40 kD; Observed band size: 55-60 kD)

Immunofluorescent analysis of c-FOS staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

FOS

UniProt

P01100, P01101, P12841

NCBI Gene ID

14281, 2353, 314322