BRCA1 Rabbit Polyclonal Antibody

SKU: orb180713

Description

BRCA1 Rabbit Polyclonal Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Molecular Biology, Protein Biochemistry

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequences identity with mouse and rat BRCA1, respectively.
• Target: Breast cancer type 1 susceptibility protein
• Molecular Weight: 290 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Breast cancer type 1 susceptibility protein; 6.3.2.-; RING finger protein 53; BRCA1; RNF53

Flow Cytometry analysis of HepG2 cells using anti-BRCA1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of BRCA1 using anti-BRCA1 antibody.Lane 1:human HeLa cell;2:human T-47D cell;3:human Raji cell.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human tonsil tissue.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human thyroid cancer tissue.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of rat brain tissue.

Quick Database Links

Gene Symbol

Breast cancer type 1 susceptibility protein

UniProt

P38398