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Catalog Number | orb180713 |
---|---|
Category | Antibodies |
Description | Anti-BRCA1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequences identity with mouse and rat BRCA1, respectively. |
Antibody Type | Primary Antibody |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 290 kDa |
UniProt ID | P38398 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Breast cancer type 1 susceptibility protein; 6.3.2 Read more... |
Note | For research use only |
Application notes | Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of HepG2 cells using anti-BRCA1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of BRCA1 using anti-BRCA1 antibody.Lane 1:human HeLa cell;2:human T-47D cell;3:human Raji cell.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human tonsil tissue.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human thyroid cancer tissue.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of mouse brain tissue.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of rat brain tissue.
ChIP, ELISA, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |