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BRCA1 Antibody
SKU: orb180713
Description
Images & Validation
−Item 1 of 8
| Tested Applications | FC, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequences identity with mouse and rat BRCA1, respectively. |
| Molecular Weight | 290 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
−Breast cancer type 1 susceptibility protein; 6.3.2.-; RING finger protein 53; BRCA1; RNF53
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Flow Cytometry analysis of HepG2 cells using anti-BRCA1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of BRCA1 using anti-BRCA1 antibody.Lane 1:human HeLa cell;2:human T-47D cell;3:human Raji cell.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human tonsil tissue.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human thyroid cancer tissue.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of mouse brain tissue.
IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of rat brain tissue.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
BRCA1 Antibody (orb180713)
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