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Catalog Number | orb316550 |
---|---|
Category | Antibodies |
Description | Anti-BMP2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human BMP-2, identical to the related mouse and rat sequences. |
Antibody Type | Primary Antibody |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 20 kDa, 40 kDa |
UniProt ID | P12643 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Bone morphogenetic protein 2; BMP-2; Bone morphoge Read more... |
Note | For research use only |
Application notes | Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human ELISA(Cap) , 1-5μg/ml, Human, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
Expiration Date | 12 months from date of receipt. |
IHC analysis of BMP-2 using anti-BMP-2 antibody. BMP-2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-BMP-2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Sandwich ELISA - Recombinant mouse BMP2 protein standard curve. Use in combination with reagents from Mouse BMP2 ELISA Kit EZ-Set (DIY Antibody Pairs).
Western blot analysis of BMP-2 using anti-BMP-2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50 ug, Lane 2: Rat Brain Tissue Lysate at 50 ug, Lane 3: U87 Whole Cell Lysate at 40 ug, Lane 4: HELA Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP-2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BMP-2 at approximately 20 kDa, 40 kDa. The expected band size for BMP-2 is at 45 kDa.
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |