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Catalog Number | orb182359 |
---|---|
Category | Antibodies |
Description | Anti-BID Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively. |
Antibody Type | Primary Antibody |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 22 kDa |
UniProt ID | P55957 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Antigen KI-67; MKI67 Read more... |
Note | For research use only |
Application notes | Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of MCF-7 cells using anti-BID antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of BID using anti-BID antibody.Lane 1:HELA Cell;2:COLO320 Cell;3:JURKAT Cell;4:SKOV Cell.
IF analysis of Bid using anti-Bid antibody. Bid was detected in immunocytochemical section of MCF-7 cells.
IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of human mammary cancer tissues.
IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of mouse spleen tissues.
IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of rat intestine tissues.
IHC analysis of BID using anti-BID antibody.BID was detected in immunocytochemical section of A549 cell.
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IH, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, IP, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |