BF-1 Antibody

• Catalog Number: orb666710
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BF-1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide of human BF-1. The exact sequence is proprietary.
• UniProt ID: P55316, Q60987, Q00939
• Tested applications: IF, WB
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FKH2; FKHL1; FKHL2; FKHL3; FKHL4; FOXG1A; FOXG1B;
• Research Area: Developmental Biology
• Note: For research use only
• Entrez: 2290, 24370, 15228
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BF-1 expression in HEK293T (A), mouse brain (B), mouse kidney (C), rat spleen (D) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 52 kD)

Immunofluorescent analysis of BF-1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.