BDNF Antibody

• Catalog Number: orb411830
• Category: Antibodies
• Description: The BDNF Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human BDNF .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human BDNF
• UniProt ID: P23363, P21237, P23560
• Tested applications: IF, WB
• Dilution range: WB: 1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Brain-derived neurotrophic factor; BDNF; Abrineuri
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 12064, 627, 24225

Western blot analysis of BDNF expression in A549 (A), mouse kidney (B) whole cell lysates. (Predicted band size: 27; 28; 29; 31; 37 kD; Observed band size: 15; 35 kD)

Immunofluorescent analysis of BDNF staining in U251MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).