BDNF Rabbit Polyclonal Antibody

SKU: orb196246

Description

BDNF Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: Anti-BDNF Antibody was raised against E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.
• Target: Neurotrophic factor BDNF precursor form
• Molecular Weight: 14 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Brain-derived neurotrophic factor; BDNF; Bdnf

Flow Cytometry analysis of U-87 cells using anti-BDNF antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of BDNF using anti-BDNF antibody.Lane 1:Rat Brain Tissue.

IHC analysis of BDNF using anti-BDNF antibody.BDNF was detected in paraffin-embedded section of Rat Brain Tissue.

IHC analysis of BDNF using anti-BDNF antibody.BDNF was detected in paraffin-embedded section of Mouse Brain Tissue.

Quick Database Links

Gene Symbol

Neurotrophic factor BDNF precursor form

UniProt

P21237