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Catalog Number | orb763014 |
---|---|
Category | Antibodies |
Description | Anti-BCR Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human BCR recombinant protein (Position: D3-N264). |
Antibody Type | Primary Antibody |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 160 kDa |
UniProt ID | P11274 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Interleukin-10; IL-10; Cytokine synthesis inhibito Read more... |
Note | For research use only |
Application notes | Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of Hela cells using anti-BCR antibody. Overlay histogram showing Hela cells (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCR Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of K562 cells using anti-BCR antibody. Overlay histogram showing K562 cells (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCR Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of BCR using anti-BCR antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCR antigen affinity purified polyclonal antibody at 0.25 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BCR at approximately 160 kDa. The expected band size for BCR is at 160 kDa.
ELISA, IHC, WB | |
Bovine, Canine, Human, Porcine | |
Goat | |
Polyclonal | |
Unconjugated |
FC, IHC-P, IP, WB | |
Bovine, Canine, Equine, Gallus, Guinea pig, Human, Mouse, Porcine, Primate, Rabbit, Rat | |
Monoclonal | |
Unconjugated |
FC | |
Bovine, Canine, Equine, Gallus, Guinea pig, Human, Mouse, Porcine, Primate, Rabbit, Rat | |
Monoclonal | |
APC |
FC | |
Bovine, Equine, Gallus, Guinea pig, Human, Mouse, Opossum, Porcine, Rabbit, Rat | |
Monoclonal | |
FITC |