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Anti-BAMBI/NMA Antibody
Catalog Number: orb623843
| Catalog Number | orb623843 |
|---|---|
| Category | Antibodies |
| Description | Anti-BAMBI/NMA Antibody. Tested in ELISA, WB applications. This antibody reacts with Human. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Purification | Immunogen affinity purified. |
| Immunogen | E.coli-derived human BAMBI/NMA recombinant protein (Position: K25-V260). |
| UniProt ID | Q13145 |
| MW | 29 kDa |
| Tested applications | ELISA, WB |
| Application notes | Western blot, 0.25-0.5μg/ml, Human ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Cross Reactivity | No cross-reactivity with other proteins. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | BMP and activin membrane-bound inhibitor homolog; Read more... |
| Note | For research use only |
Western blot analysis of BAMBI using anti-BAMBI antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAMBI antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAMBI at approximately 29 KD. The expected band size for BAMBI is at 29 KD.
