BAG1 Rabbit Polyclonal Antibody

SKU: orb669063

Description

Anti-BAG1 Antibody. Tested in WB applications. This antibody reacts with Human.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Human
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human BAG1, which shares 91.4% and 88.6% amino acid (aa) sequence identity with mouse and rat BAG1, respectively.
• Target: BAG family molecular chaperone regulator 1
• Molecular Weight: 33 kDa(S), 46 kDa(M), 56 kDa(L)
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BAG family molecular chaperone regulator 1; BAG-1; Bcl-2-associated athanogene 1; BAG1; HAP

Western blot analysis of BAG1 using anti-BAG1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAG1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAG1 at approximately 33 kDa(S), 46 kDa(M), 56 kDa(L). The expected band size for BAG1 is at 39 kDa.

Quick Database Links

Gene Symbol

BAG family molecular chaperone regulator 1

UniProt

Q99933