ATR Rabbit Polyclonal Antibody

SKU: orb865683

Description

Anti-ATR Antibody. Tested in ELISA, IF, ICC applications. This antibody reacts with Human.

Research Area

Cardiovascular Research, Epigenetics & Chromatin, Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IF
• Dilution Range: Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, -
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ATR recombinant protein (Position: E24-D1324).
• Target: Serine/threonine-protein kinase ATR
• Molecular Weight: 301 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATR; FRAP related protein 1; FRP1; MEC1; SCKL; SCKL1

IF analysis of ATR using anti-ATR antibody. ATR was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-ATR Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Quick Database Links

Gene Symbol

Serine/threonine-protein kinase ATR

UniProt

Q13535