Anti-ATP6V0B Antibody

• Catalog Number: orb1880972
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATP6V0B
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human ATP6V0B. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 533
• UniProt ID: Q99437
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Alternative names: ATP6F; V-type proton ATPase 21 kDa proteolipid sub
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATP6V0B expression in U251MG (A) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 16 kD)

Immunohistochemical analysis of ATP6V0B staining in human prostata carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.