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Anti-ATG3 Antibody

Catalog Number: orb340975

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb340975
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ATG3
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human ATG3
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:2000, IHC-P: 1:50-1:200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetATG3
Entrez64422, 171415, 67841
UniProt IDQ9CPX6, Q6AZ50, Q9NT62
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAPG3; APG3L; Ubiquitin-like-conjugating enzyme ATG
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-ATG3 Antibody

Western blot analysis of ATG3 expression in Raji (A), Hela (B), mouse liver (C), mouse heart (D) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 40 kD)

Anti-ATG3 Antibody

Immunofluorescent analysis of ATG3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • ATG3 Antibody [orb238289]

    ELISA,  IF,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
  • Anti-ATG3 Antibody [orb1473656]

    IH,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • ATG3 Antibody, Biotin conjugated [orb238290]

    ELISA

    Human

    Rabbit

    Polyclonal

    Biotin

    50 μg, 100 μg
  • ATG3 Antibody, FITC conjugated [orb238291]

    Human

    Rabbit

    Polyclonal

    FITC

    50 μg, 100 μg
  • ATG3 Antibody, HRP conjugated [orb238292]

    ELISA

    Human

    Rabbit

    Polyclonal

    HRP

    50 μg, 100 μg