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Anti-Argonaute 4/AGO4 Antibody
Catalog Number: orb371713
| Catalog Number | orb371713 |
|---|---|
| Category | Antibodies |
| Description | Anti-Argonaute 4/AGO4 Antibody. Tested in WB applications. This antibody reacts with Human, Rat. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | WB |
| Reactivity | Human, Rat |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Argonaute 4, identical to the related mouse sequence. |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 115 kDa |
| UniProt ID | Q9HCK5 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Protein argonaute-4; Argonaute4; hAgo4; Argonaute Read more... |
| Note | For research use only |
| Application notes | Western blot, 0.1-0.5μg/ml, Human, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of Argonaute 4 using anti-Argonaute 4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Argonaute 4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Argonaute 4 at approximately 115 kDa. The expected band size for Argonaute 4 is at 97 kDa.
