APEX2 Rabbit Polyclonal Antibody

SKU: orb614087

Description

Anti-APEX2 Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Monkey, Rat Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human APEX2 recombinant protein (Position: L102-A210). Human APEX2 shares 91.7% amino acid (aa) sequence identity with mouse APEX2.
• Target: DNA-
• Molecular Weight: 57 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA- (apurinic or apyrimidinic site) lyase 2; 3.1.-.-; 4.2.99.18; AP endonuclease XTH2; APEX nuclease 2; APEX nuclease-like 2; Apurinic-apyrimidinic endonuclease 2; AP endonuclease 2; APEX2; APE2, APEXL2, XTH2

Flow Cytometry analysis of A549 cells using anti-CYP7A1 antibody. Overlay histogram showing A549 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP7A1 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Western blot analysis of APEX2 using anti-APEX2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, lane 2: mouse brain tissue lysate, lane 3: HEK293 whole cell lysate, lane 4: COS-7 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APEX2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APEX2 at approximately 57 KD. The expected band size for APEX2 is at 57 KD.

Quick Database Links

Gene Symbol

DNA-

UniProt

Q9UBZ4