Angiotensin Converting Enzyme 1/Ace Antibody

SKU: orb402336

Description

Anti-Angiotensin Converting Enzyme 1/Ace Antibody. Tested in WB applications. This antibody reacts with Mouse, Rat.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Protein Biochemistry, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat Flow Cytometry(Fixed), 1-3 μg/1x10^6 cells, Mouse
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Ace, which shares 70.3% and 91.9% amino acid (aa) sequence identity with human and rat Ace, respectively.
• Target: Angiotensin-converting enzyme
• Molecular Weight: 150-180 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

ACE; ACE1; Angiotensin converting enzyme; CD143; DCP; DCP1; Dipeptidyl carboxypeptidase I; Kininase II; MVCD3

Western blot analysis of Ace using anti-Ace antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse stomach tissue lysates, Lane 4: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ace antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ace at approximately 180KD. The expected band size for Ace is at 150KD.

Quick Database Links

Gene Symbol

Angiotensin-converting enzyme

UniProt

P09470