Angiopoietin-like 4/ANGPTL4 Antibody

SKU: orb1184734

Description

Anti-Angiopoietin-like 4/ANGPTL4 Antibody. Tested in WB applications. This antibody reacts with Human.

Images & Validation

• Tested Applications: WB
• Reactivity: Human
• Application Notes:
  Western blot, 0.25-0.5 μg/ml/ml, Human. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Angiopoietin-like 4/ANGPTL4, which shares 94.7% amino acid (aa) sequence identity with mouse and rat ANGPTL4.
• Molecular Weight: 45 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Angiopoietin-related protein 4; Angiopoietin-like protein 4; Hepatic fibrinogen/angiopoietin-related protein; HFARP; ANGPTL4; ARP4, HFARP, PGAR; PP1158, PSEC0166, UNQ171/PRO197

Western blot analysis of Angiopoietin-like 4/ANGPTL4 using anti-Angiopoietin-like 4/ANGPTL4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-like 4/ANGPTL4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Angiopoietin-like 4/ANGPTL4 at approximately 45 kDa. The expected band size for Angiopoietin-like 4/ANGPTL4 is at 45 kDa.

Quick Database Links

UniProt

Q9BY76