ANAPC1 Rabbit Polyclonal Antibody

SKU: orb215266

Description

The ANAPC1 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ANAPC1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ANAPC1. The exact sequence is proprietary.
• Target: ANAPC1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TSG24; Anaphase-promoting complex subunit 1; APC1; Cyclosome subunit 1; Mitotic checkpoint regulator; Testis-specific gene 24 protein

Western blot analysis of ANAPC1 expression in HCT116 (A), PC12 (B), CT26 (C) whole cell lysates. (Predicted band size: 216 kD; Observed band size: 243 kD)

Immunofluorescent analysis of ANAPC1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

ANAPC1

UniProt

P53995, Q9H1A4

NCBI Gene ID

17222, 64682