Anti-AMPK gamma 1/2/3 Antibody

• Catalog Number: orb737247
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRKAG1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: PRKAG1; PRKAG2; PRKAG3
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: 1/500 - 1/1000, IF: 1/50 - 1/200
• Conjugation: Unconjugated
• UniProt ID: P54619
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-PRKAG1 antibody, anti-5'-AMP-activated protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AMPK gamma 1/2/3 expression in HEK293T (A), HepG2 (B), MCF7 (C), PC3 (D) whole cell lysates.

Immunofluorescent analysis of AMPK gamma 1/2/3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.