AMPK alpha 1/PRKAA1 Rabbit Polyclonal Antibody

SKU: orb413023

Description

Anti-AMPK alpha 1/PRKAA1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml ELISA, 0.1-0.5μg/ml
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human AMPK alpha 1 recombinant protein (Position: F377-R446).
• Target: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• Molecular Weight: 64 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMPK; AMPK alpha; AMPK alpha 1; AMPK subunit alpha 1; AMPK1; AMPKa1; PRKAA1

Western blot analysis of AMPK alpha 1 using anti-AMPK alpha 1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AMPK alpha 1 at approximately 64 kDa. The expected band size for AMPK alpha 1 is at 64 kDa.

Quick Database Links

Gene Symbol

5'-AMP-activated protein kinase catalytic subunit alpha-1

UniProt

Q13131