Alpha 1B-Glycoprotein/A1BG/1B Rabbit Polyclonal Antibody

SKU: orb1819501

Description

Anti-Alpha 1B-Glycoprotein/A1BG Antibody. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml, -
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E.coli-derived human IDI1 recombinant protein (Position: M1-M227). Human IDI1 shares 86.8% amino acid (aa) sequence identity with both mouse and rat IDI1.
• Target: Alpha-1B-glycoprotein
• Molecular Weight: 50-75 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

A1B; A1BG; ABG; alpha 1 B glycoprotein; Alpha 1B glycoprotein; Alpha 1B-Glycoprotein; GAB; HYST2477

Flow Cytometry analysis of HepG2 cells using anti-Alpha 1B-Glycoprotein/A1BG antibody. Overlay histogram showing HepG2 cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha 1B-Glycoprotein/A1BG Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Western blot analysis of Alpha 1B-Glycoprotein/A1BG using anti-Alpha 1B-Glycoprotein/A1BG antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: rat plasma lysates, Lane 4: mouse plasma lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1B-Glycoprotein/A1BG antigen affinity purified polyclonal antibody at 0.25 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Alpha 1B-Glycoprotein/A1BG at approximately 50-75 kDa. The expected band size for Alpha 1B-Glycoprotein/A1BG is at 54 kDa.

Quick Database Links

Gene Symbol

Alpha-1B-glycoprotein

UniProt

P04217