Cart summary

You have no items in your shopping cart.

Anti-ALKBH1 Antibody

Catalog Number: orb654308

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb654308
CategoryAntibodies
DescriptionAnti-ALKBH1 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsICC, IF, WB
ReactivityHuman
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminus of human ALKBH1, which shares 81.3% and 84.4% amino acid (aa) sequence identity with mouse and rat ALKBH1, respectively.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW40 kDa
UniProt IDQ13686
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesNucleic acid dioxygenase ALKBH1; Alkylated DNA rep
Read more...
NoteFor research use only
Application notesWestern blot, 0.1-0.25μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-ALKBH1 Antibody

Flow Cytometry analysis of A431 cells using anti-ALKBH1 antibody. Overlay histogram showing A431 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALKBH1 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-ALKBH1 Antibody

IF analysis of ALKBH1 using anti-ALKBH1 antibody. ALKBH1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-ALKBH1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-ALKBH1 Antibody

IHC analysis of ALKBH1 using anti-ALKBH1 antibody. ALKBH1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-ALKBH1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-ALKBH1 Antibody

IHC analysis of ALKBH1 using anti-ALKBH1 antibody. ALKBH1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-ALKBH1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-ALKBH1 Antibody

IHC analysis of ALKBH1 using anti-ALKBH1 antibody. ALKBH1 was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-ALKBH1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-ALKBH1 Antibody

Western blot analysis of ALKBH1 using anti-ALKBH1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALKBH1 antigen affinity purified polyclonal antibody at 0.25 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ALKBH1 at approximately 43 KD. The expected band size for ALKBH1 is at 43 KD.

  • ALKBH1 Antibody [orb353440]

    ELISA,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Anti-ALKBH1 Rabbit Monoclonal Antibody [orb866575]

    IHC,  IP,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

  • Anti-ALKBH1 Rabbit Monoclonal Antibody [orb866712]

    IHC,  WB

    Human, Mouse

    Rabbit

    Monoclonal

    Unconjugated

  • ALKBH1 Antibody [orb353441]

    ELISA,  IHC,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl
  • ALKBH1 Antibody [orb676733]

    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl