AKT Antibody

• Catalog Number: orb213545
• Category: Antibodies
• Description: The AKT Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human AKT. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human AKT. The exact sequence is proprietary.
• UniProt ID: P47196, P31749, P31750
• Tested applications: IHC, WB
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PKB; RAC; RAC-alpha serine/threonine-protein kinas
• Research Area: Apoptotic, Cancer, Developmental Biology, Epigenet
• Note: For research use only
• Entrez: 11651, 207, 24185
• Expiration Date: 12 months from date of receipt.

References

Tao, Haitao et al. Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway Clin Interv Aging, 15, 373-385 (2020)

PubMed: 32214804

Xu, Dongsheng et al. miR-10a overexpression aggravates renal ischemia-reperfusion injury associated with decreased PIK3CA expression BMC Nephrol, 21, 248 (2020)

PubMed: 32611384

Western blot analysis of AKT expression in HeLa (A), A549 (B), Raw264.7 (C), NIH3T3 (D), mouse lung (E), PC12 (F), rat heart (G) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 60 kD)

Immunohistochemical analysis of AKT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.