Anti-AIRE Antibody

• Catalog Number: orb216017
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AIRE
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AIRE. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:1000, IF/ICC: 1:100-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: AIRE
• Entrez: 11634, 326
• UniProt ID: O43918, Q9Z0E3
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti APECED antibody, anti Autoimmune regulator an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Pan, Bin et al. Interleukin-22 accelerates thymus regeneration via Stat3/Mcl-1 and decreases chronic graft-versus-host disease in mice after allotransplants Biol. Blood Marrow Transplant., (2019)

PubMed: 31195136

Western blot analysis of AIRE expression in Hela (A), rat kidney (B), rat spleen (C) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 57 kD)

Immunofluorescent analysis of AIRE staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.