ADAMDEC1 Antibody

• Catalog Number: orb234996
• Category: Antibodies
• Description: The ADAMDEC1 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ADAMDEC1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ADAMDEC1. The exact sequence is proprietary.
• UniProt ID: O15204, Q9R0X2
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ADAM DEC1; A disintegrin and metalloproteinase dom
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 58860, 27299

Western blot analysis of ADAMDEC1 expression in HEK293T (A), Hela (B), A375 (C), mouse brain (D), mouse lung (E), rat brain (F), rat lung (G) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 53 kD)

Immunofluorescent analysis of ADAMDEC1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).