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Anti-ACAT2 Antibody

Catalog Number: orb341380

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb341380
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ACAT2
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human ACAT2
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:2000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetACAT2
Entrez39, 110460, 308100
UniProt IDQ9BWD1, Q8CAY6, Q5XI22
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesACTL; Acetyl-CoA acetyltransferase, cytosolic; Ace
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-ACAT2 Antibody

Western blot analysis of ACAT2 expression in MCF7 (A), HepG2 (B), PC12 (C), mouse liver (D) whole cell lysates. (Predicted band size: 41; 44 kD; Observed band size: 41 kD)

Anti-ACAT2 Antibody

Immunohistochemical analysis of ACAT2 staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-ACAT2 Antibody

Immunofluorescent analysis of ACAT2 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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