Anti-5-HT2B Antibody

• Catalog Number: orb1289706
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to 5-HT2B
• Target: HTR2B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant protein corresponding to human 5-HT2B. The exact sequence is proprietary.
• UniProt ID: P30994, Q02152, P41595
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1:1000, IH: 1:50-1:200, IF:IC: 1:50-1:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 5-hydroxytryptamine receptor 2B; 5-HT-2B; 5-HT2B;
• Note: For research use only
• Entrez: 29581, 15559, 3357

Western blot analysis of 5-HT2B expression in SW480 (A) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 47 kD)

Immunofluorescent analysis of 5-HT2B staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.