2B4/Cd244 Rabbit Polyclonal Antibody

SKU: orb614105

Description

Anti-2B4/Cd244 Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse ELISA, 0.1-0.5μg/ml, -
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse 2B4/Cd244 recombinant protein (Position: Q20-N374).
• Target: Natural killer cell receptor 2B4
• Molecular Weight: 65-75 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2B4; CD244; h2B4; NAIL; NKR2B4; SLAMF4

Western blot analysis of Cd244 using anti-Cd244 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse RAW246.7 whole cell lysates, Lane 3: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd244 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cd244 at approximately 50 KD. The expected band size for Cd244 is at 42 KD.

Quick Database Links

Gene Symbol

Natural killer cell receptor 2B4

UniProt

Q07763