12 Lipoxygenase/ALOX12 Rabbit Polyclonal Antibody

SKU: orb215877

Description

Anti-12 Lipoxygenase/ALOX12 UBB Antibody. Tested in WB applications. This antibody reacts with Human.

Research Area

Cell Biology, Epigenetics & Chromatin, Neuroscience

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX12, different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.
• Target: Polyunsaturated fatty acid lipoxygenase ALOX12
• Molecular Weight: 75 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Polyubiquitin-B; Ubiquitin; UBB

Anti-12 Lipoxygenase antibody, Western blotting. All lanes: Anti ANOX12 at 0.5 ug/ml. Lane 1: A549 Whole Cell Lysate at 40 ug. Lane 2: MCF-7 Whole Cell Lysate at 40 ug. Lane 3: COLO320 Whole Cell Lysate at 40 ug. Lane 4: JURKAT Whole Cell Lysate at 40 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Predicted bind size: 75 KD. Observed bind size: 75 KD.

Quick Database Links

Gene Symbol

Polyunsaturated fatty acid lipoxygenase ALOX12

UniProt

P18054