ANO1 Antibody

• Catalog Number: orb1252255
• Category: Antibodies
• Description: ANO1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DG1/1485
• Tested applications: FC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG2b, kappa
• Immunogen: A recombinant human protein corresponding to amino acids 2-101 of human DOG1 was used as the immunogen for this DOG1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ANO1
• UniProt ID: Q5XXA6
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: TMEM16A Antibody: DOG1, TAOS2, ORAOV2, TMEM16A, DO
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG1 antibody (DG1/1485). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.