Annexin VI Rabbit Polyclonal Antibody

SKU: orb155670

Description

Annexin VI Rabbit Polyclonal Antibody

Research Area

Annexins, Cell Signaling

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Canine, Gallus, Human, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Annexin VI (601-673/673aa)
• Target: ANXA6
• Molecular Weight: 75 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ANX6; CBP68; CPB-II; p68; p70; AnxVI; Cabm; Camb; ANXA6_HUMAN; ANXA6; 67 kDa calelectrin; Annexin VI; Annexin-6; Calphobindin-II (CPB-II); Chromobindin-20; Lipocortin VI; Protein III; ANXA6_MOUSE; ANXA6_RAT; Calcium-binding protein 65/67 (CBP 65/67);

Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Annexin VI) Polyclonal Antibody, Unconjugated (orb155670) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Liver tissue lysates, Primary: Anti-Annexin VI (orb155670) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 67-74 kDa, Observed band size: 75 kDa.

Quick Database Links

Gene Symbol

ANXA6

UniProt

P08133