Annexin A3/ANXA3 Antibody

• Catalog Number: orb47977
• Category: Antibodies
• Description: Annexin A3/ANXA3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of rat Annexin A3 (124-141aa RQMKEISQAYYTAYKKNL), different from the related human and mouse sequences by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 36363 MW
• UniProt ID: P14669
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Annexin A3;35-alpha calcimedin;Annexin III;Annexin
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Testis Tissue Lysate, Lane 3: Rat Skeletal Muscle Tissue Lysate, Lane 4: Rat Lung Tissue Lysate, Lane 5: SW620 Cell Lysate, Lane 6: HELA Cell Lysate, Lane 7: SMMC Cell Lysate, Lane 8: M

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Mammary Cancer Tissue using Annexin A3 antibody.