Annexin A3/ANXA3 Antibody

• Catalog Number: orb259597
• Category: Antibodies
• Description: Annexin A3/ANXA3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Annexin A3 (153-184aa RKALLTLADGRRDESLKVDEHLAKQDAQILYK), different from the related mouse sequence by one amino acid, and from the related rat sequence by three amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Frozen Section), 0.5-1μg/mlImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By HeatWestern blot, 0.1-0.5μg/mlImmunocytochemistry , 0.5-1μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 36375 MW
• UniProt ID: P12429
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Annexin A3;35-alpha calcimedin;Annexin III;Annexin
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Annexin A3 using anti-Annexin A3 antibody.Lane 1:Rat Spleen tissue;2:Rat Stomach tissue;3:HELA cell;4:22RV1 cell;5:A549 cell;6:MCF-7 cell;7;SW620 Cell.

IHC analysis of Annexin A3 using anti-Annexin A3 antibody.Annexin A3 was detected in paraffin-embedded section of Human Mammary Cancer Tissue.

IHC analysis of Annexin A3 using anti-Annexin A3 antibody.Annexin A3 was detected in paraffin-embedded section of Mouse Cardiac Muscle Tissue.

IHC analysis of Annexin A3 using anti-Annexin A3 antibody.Annexin A3 was detected in paraffin-embedded section of Rat Cardiac Muscle Tissue.

IHC analysis of Annexin A3 using anti-Annexin A3 antibody.Annexin A3 was detected in frozen section of IHC(F): Human Placenta Tissue.

IHC analysis of Annexin A3 using anti-Annexin A3 antibody.Annexin A3 was detected in frozen section of IHC(F): Rat Cardiac Muscle Tissue.

IHC analysis of Annexin A3 using anti-Annexin A3 antibody.Annexin A3 was detected in frozen section of IHC(F): Mouse Cardiac Muscle Tissue.

IHC analysis of Annexin A3 using anti-Annexin A3 antibody.Annexin A3 was detected in immunocytochemical section of A549 cell.