ANKFY1 Antibody

• Catalog Number: orb1264478
• Category: Antibodies
• Description: ANKFY1 Antibody
• Target: ANKFY1
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This ANKFY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-465 amino acids from the C-terminal region of human ANKFY1.
• UniProt ID: Q9P2R3
• MW: 128 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Rabankyrin-5, Rank-5, Ankyrin repeat and FYVE doma
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: Q9P2R3

Western blot analysis in MDA-MB435, CEM, MCF-7, Jurkat, Hela cell line lysates (35 ug/lane). This demonstrates the ANKFY1 antibody detected the ANKFY1 protein (arrow).

ANKFY1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.