ANGPT1/Angiopoietin-1 Antibody

• Catalog Number: orb1538854
• Category: Antibodies
• Description: ANGPT1/Angiopoietin-1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Canine, Equine, Human, Monkey, Mouse, Rabbit
• Immunogen: Peptide with sequence C-PDFSSQKLQH, from the internal region of the protein sequence according to NP_001137.2.
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:32000), WB (1 - 2 µg/ml)
• Conjugation: Unconjugated
• Target: ANGPT1 / Angiopoietin-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: ANGPT1, Angiopoietin 1, Angiopoietin-1, AGPT, AGP1
• Note: For research use only
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing full-length Human ANGPT1 (myc and DYKDDDDK tagged), a band of approx. 48kD was observed. No bands were observed in mock-transfected HEK293 and the 48kD plus a 75kD band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 ug/ml.
• Expiration Date: 12 months from date of receipt.

Anti-ANGPT1 / Angiopoietin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human ANGPT1 with DYKDDDDK tag probed with ANGPT1 antibody (1 ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C.