ANGPT1/Angiopoietin-1 Antibody

• Catalog Number: orb1538739
• Category: Antibodies
• Description: ANGPT1/Angiopoietin-1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, IP, WB
• Reactivity: Human, Mouse
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
• Concentration: 80 mg/ml
• Dilution range: ELISA (1:5000 - 1:25000), IHC, IHC-P (1:200), IP, WB (1:500 - 1:2000)
• Conjugation: Unconjugated
• Target: ANGPT1 / Angiopoietin-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Alternative names: ANGPT1, Angiopoietin 1, Angiopoietin-1, AGPT, AGP1
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B62 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B62 was determined to be 1:200.
• Expiration Date: 12 months from date of receipt.