Angiotensin Converting Enzyme 1/ACE Antibody

• Catalog Number: orb215879
• Category: Antibodies
• Description: Angiotensin Converting Enzyme 1/ACE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ACE recombinant protein (Position: K651-Y864). Human ACE shares 73% and 76% amino acid (aa) sequences identity with mouse and rat ACE, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, MouseImmunocytochemistry/Immunofluorescence, 2μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 149715 MW
• UniProt ID: P12821
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Angiotensin-converting enzyme;ACE;3.2.1.-;3.4.15.1
• Note: For research use only
• Application notes: WB: The detection limit for ACE is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of ACE using anti-ACE antibody.Lane 1:rat lung tissue;2:mouse lung tissue;3:human Raji cell.

IF analysis of ACE using anti-ACE antibody.ACE was detected in immunocytochemical section of A431 cell.

IHC analysis of ACE using anti-ACE antibody.ACE was detected in paraffin-embedded section of human placenta tissue.

IHC analysis of ACE using anti-ACE antibody.ACE was detected in paraffin-embedded section of mouse lung tissue.

IHC analysis of ACE using anti-ACE antibody.ACE was detected in paraffin-embedded section of rat lung tissue.

IHC analysis of ACE using anti-ACE antibody.ACE was detected in paraffin-embedded section of rat lung tissue.

IHC analysis of ACE using anti-ACE antibody.ACE was detected in frozen section of human placenta tissues.

IHC analysis of ACE using anti-ACE antibody.ACE was detected in frozen section of mouse lung tissues.