AMPK2 Antibody (Center)

SKU: orb1929288

This product can be custom made

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution Range	WB - 1:1000
Reactivity	Human
Predicted Reactivity	Mouse, Porcine, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	This AMPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the Central region of human AMPK2. Antigen Region: 265-294 aa.
Target	PRKAA2 (HGNC:9377)
Molecular Weight	62320 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis of AMPK2 Antibody (Center) in Hela cell line lysates (35 ug/lane).AMPK2 (arrow) was detected using the purified Pab.

Western blot analysis of AMPK2 (arrow) using AMPK2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA2 (Center) gene (Lane 2).

Anti-AMPK2 Antibody (Center) at 1:1000 dilution + 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 62 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

PRKAA2 (HGNC:9377)

UniProt

P54646

RefSeq (Protein):NP_006243.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006243.2

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Protocol Information

Western Blot (IB, immunoblot)

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