AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody

• Catalog Number: orb1415133
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AMPKα1/2 (phospho Thr183/172).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Dilution range: IF: 1:50-200, WB: 1/500 - 1/2000, IHC: 1/100 - 1/300, E: 1/40000
• Conjugation: Unconjugated
• UniProt ID: Q13131, P54646
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: PRKAA1; AMPK1; 5'-AMP-activated protein kinase cat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of KB cells using Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody diluted at 1: 500

Western Blot analysis of various cells using Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody diluted at 1: 500

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1: 100 (4°C, overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western Blot analysis of mouse-lung cells using primary antibody diluted at 1: 1000 (4°C overnight). Secondary antibody: Goat Anti-rabbit IgG IRDye 800 (diluted at 1: 5000, 25°C, 1 hour). Cell lysate was extracted by Minute Plasma Membrane Protein Isolation and Cell Fractionation Kit.

Immunofluorescence analysis of rat-heart tissue. 1, AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody (red) was diluted at 1: 200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1: 300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B