AMPK alpha 1/2 antibody

• Catalog Number: orb667799
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AMPK alpha 1/2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human AMPK alpha 1/2. The exact sequence is proprietary.
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PRKAA1; PRKAA2
• Entrez: 5563, 108079, 105787, 65248, 78975, 5562
• UniProt ID: Q5EG47, P54646, Q13131, P54645, Q8BRK8, Q09137
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-PRKAA1 antibody, anti-AMPK1 antibody, anti-5'
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AMPK alpha 1/2 expression in HeLa (A), mouse brain (B), rat heart (C) whole cell lysates. (Predicted band size: 64; 65 kD; Observed band size: 62 kD)

Immunofluorescent analysis of AMPK alpha 1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.