AMPK alpha-1 Rabbit Polyclonal Antibody

SKU: orb1602462

Description

AMPK alpha-1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human AMPK alpha-1 protein (1-207/559aa)
• Target: PRKAA1
• Molecular Weight: 64 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

AMPK alpha-1; 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; AAPK1; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP-activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK 1; AMPK alpha 1 chain; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase; hormone sensitive lipase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; wu:fa94c10; AAPK1_HUMAN; AMPKα1; AMPK α1; AMPKα 1; AMPK α 1; AMPK AMPK-α1; AMPK-α-1; α-1; AMPK-a; AMPK a1.

Measured by its binding ability in a indirect ELISA. Immobilized Human AMPK alpha-1 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human AMPK alpha-1 Antibody, the EC50 is 13.01 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human AMPK alpha-1 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human AMPK alpha-1 Antibody, the EC50 is 133.7 ng/ml.

Sample: Lane 1: Human HUVEC cell lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-AMPK alpha-1 (orb1602462) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kDa, Observed band size: 64 kDa.

Quick Database Links

Gene Symbol

PRKAA1