AMPK alpha-1 Rabbit Polyclonal Antibody

SKU: orb1602462

Description

AMPK alpha-1 Rabbit Polyclonal Antibody

Research Area

Alzheimer's disease, Cancer Research, Cell Biology, Energy Metabolism, Fatty Acid Oxidation, Fatty Acids, Hypoxia, Kinases, Metabolism, Neurodegenerative Disease, Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB=1:500-2000, ELISA=1:5000-10000
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human AMPK alpha-1 protein (1-207/559aa)
• Target: PRKAA1
• Molecular Weight: 64 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMPKalpha1; C130083N04Rik; AAPK1_MOUSE; Prkaa1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase (ACACA kinase); Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase); Tau-protein kinase PRKAA1; 2.7.11.1; AAPK1_RAT; Ampk1; AMPK; AMPK alpha 1; AMPKa1;

Measured by its binding ability in a indirect ELISA. Immobilized Human AMPK alpha-1 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human AMPK alpha-1 Antibody, the EC50 is 13.01 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human AMPK alpha-1 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human AMPK alpha-1 Antibody, the EC50 is 133.7 ng/ml.

Sample: Lane 1: Human HUVEC cell lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-AMPK alpha-1 (orb1602462) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kDa, Observed band size: 64 kDa.

Quick Database Links

Gene Symbol

PRKAA1